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Image Search Results
Journal: Biochemical and biophysical research communications
Article Title: DCAF7 is required for maintaining the cellular levels of ERCC1-XPF and nucleotide excision repair
doi: 10.1016/j.bbrc.2019.08.147
Figure Lengend Snippet: (A and B) DCAF7-Myc and/or FLAG-ERCC1 were ectopically expressed in XP2YO(SV) cells and cell lysates were immunoprecipitated by anti-FLAG antibody resin (A) or anti-Myc antibody resin (B). (C and D) FLAG-DCAF7 and/or Myc-tagged XPF (C) or non-tagged XPF (D) were expressed in XP2YO(SV) cells and precipitated by either anti-Myc (C) or anti-FLAG (D) antibody resin. (E) The same amounts of Non-tagged ERCC1 and FLAG-DCAF7 expression plasmids (0.65 μg each) were co-transfected with an increasing amount (0.65–5.2 μg) of XPF-Myc expression plasmid into XP2YO(SV) cells. The total amount of DNA was adjusted with empty vector pEF6/Myc-His. After 48 h, cell lysates were prepared and immunoprecipitated by anti-FLAG antibody resin. Each sample was analyzed by western blotting with the indicated antibodies.
Article Snippet: We also used home-made monoclonal
Techniques: Immunoprecipitation, Expressing, Transfection, Plasmid Preparation, Western Blot
Journal: Biochemical and biophysical research communications
Article Title: DCAF7 is required for maintaining the cellular levels of ERCC1-XPF and nucleotide excision repair
doi: 10.1016/j.bbrc.2019.08.147
Figure Lengend Snippet: (A) U2OS cells were transfected with DCAF7 siRNA (#1) and incubated for the indicated period. After cell lysis, each sample was analyzed by western blotting with the indicated antibodies (left panel). The signal intensity of each band was quantified and adjusted using internal control β-actin (right panel). (B - E) U2OS cells were transfected with either control siRNA or DCAF7 siRNA (#1) (B) or TCP1α siRNA (#1) (D). After 72 h, cell lysates were prepared and analyzed by western blotting with the indicated antibodies. An asterisk indicates non-specific bands. The impacts of DCAF7 or TCP1α knockdown on ERCC1-XPF level were tested three times, and the mean and SD were shown in (C) or (E), respectively.
Article Snippet: We also used home-made monoclonal
Techniques: Transfection, Incubation, Lysis, Western Blot, Control, Knockdown